Inclusion Criteria: 

• Healthy adults aged 18-35 years.
• Absence of any significant medical or psychiatric disease (as per the investigators’ judgment).
• Nonsmoker.
• Nonpregnant.
• History of normal sleep duration (reported average sleep duration between 7-9 hours/night and no habitual napping).
• Both biological parents receiving care at healthcare facilities affiliated with Mayo Clinic Rochester.
• Ability to understand study procedures and to comply with them for the entire length of the study.
• Ability to understand English and provide informed consent.

Exclusion Criteria:

• Age < 18 or > 35 years.
• Body mass index < 18.5 or ≥ 30 kg/m2.
• Pregnant or lactating.
• Unable to determine history of HTN in participant’s biological parents or subject being adopted.
• Use of tobacco, marijuana products or vaping.
• Excessive caffeine consumption (> 400 mg/day).
• Excessive alcohol consumption (> 7 drinks/week for women, > 14 drinks/week for men).
• Office SBP/DBP ≥ 130/80 mmHg.
• Daytime ambulatory SBP/DBP ≥ 130/80 mmHg.
• Fasting glucose ≥ 126 mg/dl.
• Glomerular filtration rate < 60 mL/min/BSA.
• History of significant medical or psychiatric disorders (as per the investigators’ judgment).
• Regular use of prescription medications other than contraceptives.
• Use of melatonin supplements or any other over-the-counter sleep aid.
• Moderate-to-severe sleep disordered breathing (apnea/hypopnea index, AHI ≥ 15) or mild sleep disordered breathing (AHI 5-15) with daytime sleepiness (Epworth Sleepiness Scale > 10).
• Moderate-to-severe insomnia (Insomnia Severity Index > 14).
• Restless leg syndrome (as per the Cambridge-Hopkins questionnaire).
• Excessive daytime sleepiness (Epworth Sleepiness Scale > 15).
• Extreme chronotype (Morningness-Eveningness Questionnaire > 69 or < 31).
• Night shift work.
• Habitual high levels of physical activity (> 3000 MET×minutes/week)125.
• Ongoing participation in other research studies (as per the investigators’ judgment).
• Any other medical, geographic, or social factor making study participation impractical.
• Not English-speaking and/or inability to provide informed consent.
• Exclusionary for blood draws: hemoglobin < 12 g/dL in women / < 13.5 g/dL in men; participants with measured hemoglobin levels below these thresholds will be allowed to continue participate in study tests and procedures not involving blood draws.

Note: Other protocol defined Inclusion/Exclusion Criteria may apply.

Eligibility last updated 2/21/2024. Questions regarding updates should be directed to the study team contact.