Clinical Trials

Inclusion Criteria

• Body mass index (BMI) 19-30 kg/m2
• Age 18-45 years
• Availability of transportation
• Ability to sign informed consent form

Exclusion Criteria

• Medication or supplements (i.e. amino acids, protein) known to affect protein metabolism
• Presence of acute illness
• History of liver disease
• Uncontrolled metabolic disease, including renal disease
• Atrial fibrillation, history of syncope, limiting or unstable angina, or congestive heart failure
• Chronically elevated blood pressure with systolic, >140 mmHg or diastolic >100 mmHg
• Cardiac pacemaker or other medical device implanted in the body
• ECG documented abnormalities such as >0.2 mV horizontal or downsloping ST-segment depression, frequent arrhythmias of >10 premature ventricular contractions/min, or valvular disease
• Low hemoglobin or hematocrit with 2-h plasma glucose >200 mg/dl in the OGTT
• Current participation in a weight-loss regimen, including extreme dietary practices
• Smoking
• Use of anabolic steroids or corticosteroids within 3 months
• Any other condition or event considered exclusionary by the PI and the study physician