Services

Next-generation sequencing

Next-generation sequencing is the process of sequencing DNA or RNA by producing millions to billions of sequencing reads in a massively parallel manner. Also known as deep sequencing, next-generation sequencing allows for the sequencing of the entire genome, exome, or transcriptome. This makes it possible to identify complex genetic differences that may cause disease or help diagnose and treat disease. The Genome Analysis Core offers a full range of services and applications for next-generation sequencing, including:

  • Whole-genome sequencing (short and long read).
  • Whole-genome methylation.
  • RNA sequencing.
  • Targeted sequencing (QIAseq).
  • Small RNA (microRNA).
  • 10x Genomics single cell RNA sequencing.
  • Kinnex full-length and single cell RNA long-read.
  • Olink.

Due to the complexity of next-generation sequencing, the Genome Analysis Core recommends a consultation to properly plan a new research project.

Equipment

  • Illumina NovaSeq X Plus Next-Generation DNA Sequencer.
  • Illumina NovaSeq 6000 Next-Generation DNA Sequencers (2).
  • Illumina NextSeq 2000 Next-Generation DNA Sequencers (3).
  • Illumina MiSeq Personal Sequencers (3).
  • Agilent Bravo liquid handlers for automated library prep (2).
  • Perkin Elmer SciClone liquid handlers for automated library prep (4).
  • Perkin Elmer Janus liquid handlers for automated library prep (2).
  • Hamilton Star liquid handler for automated library prep (2).
  • Hamilton Starlet liquid handler for automated prep.
  • Sage Science Blue Pippin Preps (2) for Automated DNA fragment size selection.
  • PacBio Revio Sequencing System.
  • Oxford Nanopore Technologies PromethION 2 Solo.
  • 10x Chromium Single-Cell Systems (3).

Genotyping

The Genome Analysis Core at Mayo Clinic has over a decade of experience providing genomics support for both large and small projects conducted by Mayo Clinic investigators and their collaborators. The core also provides consultations to assist investigators in determining how best to achieve their aims.

Equipment

  • Tecan Roma (3) liquid handlers for Illumina Infinium BeadArray assays with autoloaders (2).
  • Illumina iScan (4) scanners for Illumina Infinium BeadArray assays.