Targeted Metabolomics
The Mayo Clinic Metabolomics Core has established a large number of targeted panels for quantitation of selected groups of metabolites using mass spectrometry. The key distinction between untargeted and targeted measurements is that these targeted panels provide accurate absolute quantitation of a focused group of analytes using available standards.
Targeted metabolomics is a quantitative approach in which a known set of metabolites is quantitated using 13C, 2H or 15N isotope-labeled internal or external reference compounds. The resulting data can then be used as input variables for statistical analysis.
The Metabolomics Core's panels include many metabolites of intermediary metabolism, lipids, carboxylic acids, organic acids, neuromodulators and drug metabolites. These types of panels are established to meet the needs of investigators on a case-by-case basis. Investigators who are interested in measuring metabolites not listed below should contact the core director to discuss possibilities for establishing a method.
Amino acids (AA) panel: 42 analytes
| Histidine |
Hydroxyproline |
1-methylhistidine |
3-methylhistidine |
| Asparagine |
Phosphoethanolamine |
Arginine |
Carnosine |
| Taurine |
Anserine |
Serine |
Glutamine |
| Ethanolamine |
Glycine |
Aspartic acid |
Sarcosine |
| Citrulline |
Glutamic acid |
Beta-alanine |
Threonine |
| Alanine |
Gamma-amino-n-butyric acid |
Alpha-aminoadipic acid |
Beta-aminoisobutyric acid |
| Proline |
Hydroxylysine 1 |
Hydroxylysine 2 |
Alpha-amino-n-butyric acid |
| Ornithine |
Cystathionine 1 |
Cystathionine 2 |
Lysine |
| Cysteine |
Tyrosine |
Methionine |
Valine |
| Isoleucine |
Allo-isoleucine |
Homocysteine |
Leucine |
| Phenylalanine |
Tryptophan |
|
|
Neuromodulators (NM) panel: 13 analytes
| Acetylcholine |
Adenosine |
Histidine |
Serine |
| Taurine |
Glutamine |
Glycine |
Aspartic acid |
| Glutamic acid |
Gamma-amino-n-butyric acid |
Norepinephrine |
Dopamine |
| Serotonin |
|
|
|
Tricarboxylic acids (TCA) panel: 12 analytes
| Lactic acid |
Fumaric acid |
Succinic acid |
Oxaloacetic acid |
| Alpha-ketoglutaric acid |
Malic acid |
Cis aconitic acid |
Citric acid |
| Isocitric acid |
Glutamic acid |
2-hydroxyglutarate |
Aspartate |
Nonesterified fatty acids (NEFA) panel: 12 analytes
| Myristic acid |
Palmitic acid |
Palmitoleic acid |
Palmitelaidic acid |
| Stearic acid |
Oleic acid |
Elaidic acid |
Linoleic acid |
| Alpha-linolenic acid |
Arachidonic acid |
Eicosapentaenoic acid |
Docosahexaenoic acid |
Short-chain fatty acids (SCFA) panel: 8 analytes
| Acetic acid |
Propionic acid |
Isobutyric acid |
Butyric acid |
| Valeric acid |
Isovaleric acid |
Isocaproic acid |
Hexanoic acid |
Sphingolipids panel: 12 analytes
| Sphingosine |
Sphinganine |
Sphingosine-1-phosphate |
18C8-ceramide |
| 18C14-ceramide |
18C16-ceramide |
18C18:1-ceramide |
18C18-ceramide |
| 18C20-ceramide |
18C22-ceramide |
18C24:1-ceramide |
18C24-ceramide |
Hexosylceramides panel: 14 analytes
| Galactosyl(beta) sphingosine |
C12-galactosyl(beta) ceramide |
C16-galactosyl(beta) ceramide |
C18-galactosyl(beta) ceramide |
| C18:1-galactosyl(beta) ceramide |
C24-galactosyl(beta) ceramide |
C24:1-galactosyl(beta) ceramide |
Glucosyl(beta) sphingosine |
| C12-glucosyl(beta) sphingosine |
C16-glucosyl(beta) sphingosine |
C18-glucosyl(beta) sphingosine |
C18:1-glucosyl(beta) sphingosine |
| C24-glucosyl(beta) sphingosine |
C24:1-glucosyl(beta) sphingosine |
|
|
Diacylglycerols (DAG) panel: 13 analytes
| 1,2-12:0/12:0 DAG |
1,2-14:0/14:0 DAG |
1,2-16:0/16:0 DAG |
1,2-16:0/18:2 DAG |
| 1,2-16:0/18:1 DAG |
1,2-18:0/16:0 DAG |
1,2-18:1/18:2 DAG |
1,2-18:1/18:1 DAG |
| 1,2-18:0/18:2 DAG |
1,2-18:0/18:0 DAG |
1,3-18:2/18:2 DAG |
1,2-18:0/20:4 DAG |
| 1,2-18:0/22:6 DAG |
|
|
|
Acylcarnitines panel: 14 analytes
| Carnitine |
Acetylcarnitine |
Propionylcarnitine |
Isobutyrylcarnitine |
| Butyrylcarnitine |
Isovalerylcarnitine |
Octanoylcarnitine |
Decanoylcarnitine |
| Lauroylcarnitine |
Myristoylcarnitine |
Palmitoylcarnitine |
Linoleoylcarnitine |
| Oleoylcarnitine |
Stearoylcarnitine |
|
|
Trimethylamine (TMAO) panel: 5 analytes
| Carnitine |
Trimethylamine |
Trimethylamine n-oxide |
Choline |
| Betaine |
|
|
|
Bile acids panel: 18 analytes
| Cholic acid |
Chenodeoxycholic acid |
Deoxycholic acid |
Glycocholic acid |
| Glycochenodeoxycholic acid |
Glycodeoxycholic acid |
Glycolithocholic acid |
Glycoursodeoxycholic acid |
| Hyodeoxycholic acid |
Lithocholic acid |
Alpha+omega-muricholic acid |
Beta-muricholic acid |
| Taurocholic acid |
Taurochenodeoxycholic acid |
Taurodeoxycholic acid |
Taurolithocholic acid |
| Tauroursodeoxycholic acid |
Ursodeoxycholic acid |
|
|
Endocannabinoids panel: 4 analytes
| 2-arachidonoyl glycerol |
Arachidonoylethanolamide |
| Oleoylethanolamide |
Palmitoylethanolamide |
Alpha-hydroxyglutaric acid isomers: 2 analytes
| D-alpha-hydroxyglutaric acid |
L-alpha-hydroxyglutaric acid |
Ketoacids panel: 3 analytes
| Alpha-ketoisocaproic acid |
Alpha-keto-beta-methylvaleric acid |
Alpha-ketoisovaleric acid |
Individual drug target
| Metformin |
Acamprosate |
Valproic acid |
Nicardipine |
| Nimodipine |
Acesulfame potassium |
Tryptamine |
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The Metabolomics Core also has a Bruker IVDr platform for automation analysis of human plasma and urine samples. Visit the Bruker website to learn more.