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Germ-free murine isolators
The germ-free murine facility allows lab members to deconstruct a complex system and mechanistically address the role of gut bacteria in maintaining health and causing disease.
Individualized Responses of Gut Microbiome to Diet
This diagram describes the interaction between diet, gut microbiota and gastrointestinal motility.
This diagram describes the interaction between diet, gut microbiota and gastrointestinal motility.
Individualized dietary responses
Gut microbiota from three healthy individuals modeled in germ-free mice produce different metabolites following a similar dietary intervention. This image was published as part of a larger figure in Smits SA, et al. Individualized responses of gut microbiota to dietary intervention modeled in humanized mice. mSystems. 2016;1:e00098. In the video below, Dr. Kashyap provides a summary of findings.
Dr. Kashyap's Gut Microbiome Lab is investigating the effect of an individual's microbiome in the response to dietary interventions and the effects of diet-induced changes in the gut microbiome on gastrointestinal physiology. To do this, the team is examining how environmental factors, including diet, can modify the relationship between gastrointestinal motility and the gut microbiota using a germ-free murine model system.
Scientists know that diet may impact motility directly, via changes in the bulk flow of material through the gut, or indirectly, through an impact on the microbiota. Dietary polysaccharides are a major fuel for the distal gut microbial community; thus, gut microbiomes have a large repertoire of carbohydrate-degrading genes. Dietary fermentable polysaccharides can alter microbiota composition, in part, because community members differ in their ability to metabolize them.
Additionally, different bacteria produce different fermentation end products when metabolizing a specific carbohydrate. Changes in production of short-chain fatty acids, such as butyrate, can influence diverse aspects of host physiology, including motility.
A part of this project is examining the relationship between diet, gut microbiota and gastrointestinal physiology to better understand responses in diseases such as irritable bowel syndrome (IBS) and obesity. Patients with IBS often report food sensitivities and avoid one or more food groups that seem to worsen their symptoms. The Gut Microbiome Lab seeks to develop individualized dietary therapies for management of gastrointestinal motility disorders such as IBS and for metabolic disorders such as obesity.
Diet and gut microbiota
Dr. Kashyap provides a summary of the research published in "Individualized responses of gut microbiota to dietary intervention modeled in humanized mice" by Samuel A. Smits, Angela Marcobal, Steven Higginbottom, Justin L. Sonnenburg and Purna C. Kashyap.